Label-free and sensitive thrombin sensing on a molecularly grafted aptamer on graphene.
نویسندگان
چکیده
A unique nanocomposite was constructed by grafting an aptamer on graphene and used as a label-free biosensor for thrombin sensing, showing good selectivity, a low detection limit of 0.45 fM and high sensitivity, with a linear response up to 100 fM.
منابع مشابه
A novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes
Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-co...
متن کاملTime-resolved fluorescence aptamer-based sandwich assay for thrombin detection.
In the present study, the authors report a novel sensitive method for the detection of thrombin using time-resolved fluorescence sensing platform based on two different thrombin aptamers. The thrombin 15-mer aptamer as a capture probe was covalently attached to the surface of glass slide, and the thrombin 29-mer aptamer was fluorescently labeled as a detection probe. A bifunctional europium com...
متن کاملLabel-free fluorescent detection of thrombin using G-quadruplex-based DNAzyme as sensing platform.
We report herein a label-free and sensitive fluorescent method for detection of thrombin using a G-quadruplex-based DNAzyme as the sensing platform. The thrombin-binding aptamer (TBA) is able to bind hemin to form the G-quadruplex-based DNAzyme, and thrombin can significantly enhance the activity of the G-quadruplex-based DNAzyme. The G-quadruplex-based DNAzyme is found to effectively catalyze ...
متن کاملA novel microfluidic chip electrophoresis strategy for simultaneous, label-free, multi-protein detection based on a graphene energy transfer biosensor.
A new type of high-throughput and parallel optical sensing platform with a single-color probe based on microfluidic chip electrophoresis combined with aptamer-carboxyfluorescein/graphene oxide energy transfer is reported here. Label-free protein multi-targets were detected, even in challenging complex samples without any pre-treatment.
متن کاملAptamer-functionalized graphene-gold nanocomposites for label-free detection of dielectrophoretic-enriched neuropeptide Y
Article history: Received 19 May 2016 Received in revised form 31 July 2016 Accepted 15 September 2016 Available online 16 September 2016 We present an electrokinetically enhanced aptamer sensing platform on a disposable plastic chip for label-free detection of neuropeptide Y (NPY), which is a key neurological biomarker. The sensor consists of aptamerfunctionalized graphene-gold nanocomposites ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 48 5 شماره
صفحات -
تاریخ انتشار 2012